POLA RESISTENSI ANTIBIOTIK Escherihia Coli YANG DIISOLASI DARI PRODUK ASAL UNGGAS
Keywords:
resistensi antibiotika, multidrug resistance, Escherichia coli, hati ayam
Abstract
Perkembangan resistensi antibiotika pada E. coli yang ditemukan pada produk ternak mengancam keamanan pangan asal hewan. Penelitian ini bertujuan untuk mengetahui pola resistensi E. coli yang di isolasi dari produk unggas pada tingkat ritel. Penelitian ini melakukan isolasi dan identifikasi E. coli dari 30 sampel hati ayam yang berasal dari dua pasar tradisional di kota Medan, Sumatera Utara. Uji resistensi antibiotika dilakukan dengan menggunakan metode difusi cakram. Data resistensi antibiotika dan multid-drug resistance dianalisis dengan menggunakan WHONET 5.6. Hasil penelitian ini menunjukkan bahwa sebanyak 23.3% sampel hati ayam terkontaminasi oleh E. coli. Hasil uji resistensi antibiotik menunjukkan bahwa isolat E coli resisten terhadap ampicillin (100%), tetrasiklin (71%) dan ciprofloksasin (14%). Prevalensi MDR yang relatif tinggi yaitu sebesar 42.9% ditemukan pada studi ini. Indeks MAR yang diperoleh adalah antara 0.3-0.7 dengan rata-rata MAR adalah 0.3 pada tujuh isolat E coli.
References
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Saud, B., Paudel, G., Khichaju, S., Bajracharya, D., Dhungana, G., Awasthi, M. S., & Shrestha, V. (2019). Multidrug-resistant bacteria from raw meat of buffalo and chicken, Nepal. Veterinary Medicine International, 2019. https://doi.org/10.1155/2019/7960268
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Sudarmadi, A. A. M., Prajitno, S., & Widodo, A. D. W. (2020). Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia. Biomolecular and Health Science Journal, 3(2), 80. https://doi.org/10.20473/bhsj.v3i2.22170
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Akanbi, O. B., Olorunshola, I. D., Osilojo, P., Ademola, E., Agada, G., Aiyedun, J., Odita, C. I., & Ola-Fadunsin, S. D. (2022). Escherichia Coli Infections, and Antimicrobial Resistance in Poultry Flocks, in North Central Nigeria. Media Kedokteran Hewan, 33(3), 188–207. https://doi.org/10.20473/mkh.v33i3.2022.188-207
Bengtsson, B., & Greko, C. (2014). Antibiotic resistance-consequences for animal health, welfare, and food production. Upsala Journal of Medical Sciences, 119(2), 96–102. https://doi.org/10.3109/03009734.2014.901445
Chantziaras, I., Boyen, F., Callens, B., & Dewulf, J. (2014). Correlation between veterinary antimicrobial use and antimicrobial resistance in food-producing animals: A report on seven countries. Journal of Antimicrobial Chemotherapy, 69(3), 827–834. https://doi.org/10.1093/jac/dkt443
CIVAS. (2021). Penyalahgunaan Antibiotik di Peternakan Ayam Broiler. Penyalahgunaan Antibiotik Di Peternakan Ayam Broiler. http://civas.net/2021/07/16/penyalahgunaan-antibiotik-di-peternakan-ayam-broiler/
CLSI. (2018). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Test for bacteria Isolated from Animals (5th ed.). CLSI supplement VET01S. Wayne, PA: Clinical and Laboratory Standards Institute.
Davis, G. S., Waits, K., Nordstrom, L., Grande, H., Weaver, B., Papp, K., Horwinski, J., Koch, B., Hungate, B. A., Liu, C. M., & Price, L. B. (2018). Antibiotic-resistant Escherichia coli from retail poultry meat with different antibiotic use claims. BMC Microbiology, 18(1), 1–7. https://doi.org/10.1186/s12866-018-1322-5
Effendi, M. H., Cicilia, R., Rahmahani, J., & Tyasningsih, W. (2020). Public Awareness for Antimicrobial Resistance from Escherichia coli Isolated from Beef Sold on Several Wet Market in Surabaya, Indone. Indian Journal of Public Health, 11(9), 295–300.
García‐béjar, B., de Blas Martín, I. G., Arévalo‐villena, M., & Pérez, A. B. (2021). High prevalence of antibiotic‐resistant escherichia coli isolates from retail poultry products in spain. Animals, 11(11). https://doi.org/10.3390/ani11113197
González-Gutiérrez, M., García-Fernández, C., Alonso-Calleja, C., & Capita, R. (2020). Microbial load and antibiotic resistance in raw beef preparations from northwest Spain. Food Science and Nutrition, 8(2), 777–785. https://doi.org/10.1002/fsn3.1319
Gustiani, E., & Fahmi, T. (2022). Peran Sektor Peternakan Mendukung Ketahanan Pangan Di Era New Normal Melalui Penerapan Teknologi Reproduksi …. Prosiding Seminar Nasional Hasil Penelitian Agribisnis, 70–76.
Hassani, S., Moosavy, M. H., Gharajalar, S. N., Khatibi, S. A., Hajibemani, A., & Barabadi, Z. (2022). High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk. Scientific Reports, 12(1), 1–10. https://doi.org/10.1038/s41598-022-07845-6
Jang, S., Biberstein, E., & Hirsh, D. (2008). A Diagnostic: Manual of Veterinary Clinical Bacteriology and Mycology. Iowa: University of California Davis County.
Januari, C., Sudarwanto, M. B., & Purnawarman, T. (2019). Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (Antibiotic Resistance In Escherichia Coli Isolated From Chicken Meat Of Traditional Markets In The City Of Bogor). Jurnal Veteriner, 20(1), 125. https://doi.org/10.19087/jveteriner.2019.20.1.125
Kapena, M. S., Muma, J. B., Mubita, C. M., & Munyeme, M. (2020). Antimicrobial resistance of Escherichia coli and Salmonella in raw retail table eggs in Lusaka, Zambia. Veterinary World, 13(11), 2528–2533. https://doi.org/10.14202/VETWORLD.2020.2528-2533
Karnadi, A. (2022). Produksi Ayam Pedaging Capai 3,43 Juta Ton pada 2021. Data Indonesia. https://dataindonesia.id/sektor-riil/detail/produksi-ayam-pedaging-capai-343-juta-ton-pada-2021
Ma, F., Xu, S., Tang, Z., Li, Z., & Zhang, L. (2021). Use of antimicrobials in food animals and impact of transmission of antimicrobial resistance on humans. Biosafety and Health, 3(1), 32–38. https://doi.org/10.1016/j.bsheal.2020.09.004
Martin, N. H., Trmcic, A., Hsieh, T. H., Boor, K. J., & Wiedmann, M. (2016). The evolving role of coliforms as indicators of unhygienic processing conditions in dairy foods. Frontiers in Microbiology, 7(SEP), 1–8. https://doi.org/10.3389/fmicb.2016.01549
Nhung, N. T., Cuong, N. V., Thwaites, G., & Carrique-Mas, J. (2016). Antimicrobial usage and antimicrobial resistance in animal production in Southeast Asia: A review. Antibiotics, 5(4), 1–24. https://doi.org/10.3390/antibiotics5040037
Rahmaniar, R. P., Widhowati, D., & Hidayah, N. (2022). Resistansi Antibiotik Secara Fenotip Dan Deteksi Gen TetA pada Sampel Hati Ayam di Pasar Dukuh Kupang Surabaya. Jurnal Ilmu Peternakan Dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science), 11(3), 284. https://doi.org/10.46549/jipvet.v11i3.244
Ranasinghe, R. A. S. S., Satharasinghe, D. A., Anwarama, P. S., Parakatawella, P. M. S. D. K., Jayasooriya, L. J. P. A. P., Ranasinghe, R. M. S. B. K., Rajapakse, R. P. V. J., Huat, J. T. Y., Rukayadi, Y., Nakaguchi, Y., Nishibuchi, M., Radu, S., & Premarathne, J. M. K. J. K. (2022). Prevalence and Antimicrobial Resistance of Escherichia coli in Chicken Meat and Edible Poultry Organs Collected from Retail Shops and Supermarkets of North Western Province in Sri Lanka. Journal of Food Quality, 2022. https://doi.org/10.1155/2022/8962698
Roy, P., Purushothaman, V., Koteeswaran, A., & Dhillon, A. S. (2006). Isolation, characterization, and antimicrobial drug resistance pattern of Escherichia coli isolated from Japanese quail and their environment. Journal of Applied Poultry Research, 15(3), 442–446. https://doi.org/10.1093/japr/15.3.442
Ružauskas, M., Šiugždiniene, R., Krikštolaitis, R., Virgailis, M., & Zienius, D. (2010). Prevalence and antimicrobial resistance of E. coli isolated from chicken liver sold in retail markets. Veterinarija Ir Zootechnika, 52(74), 67–72.
Saud, B., Paudel, G., Khichaju, S., Bajracharya, D., Dhungana, G., Awasthi, M. S., & Shrestha, V. (2019). Multidrug-resistant bacteria from raw meat of buffalo and chicken, Nepal. Veterinary Medicine International, 2019. https://doi.org/10.1155/2019/7960268
Suady, I. (2019). AMU-AMR Surveillance System in Indonesia on Livestock and Animal Health Sector : approach & findings Imron Suandy Poultry Population in Indonesia in 2013 - 2017. https://rr-asia.woah.org/wp-content/uploads/2020/01/11-amu-amr-surveillance-indonesia_saundy.pdf
Sudarmadi, A. A. M., Prajitno, S., & Widodo, A. D. W. (2020). Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia. Biomolecular and Health Science Journal, 3(2), 80. https://doi.org/10.20473/bhsj.v3i2.22170
Syahputri, S. A. H., Suswaty, E., Nurdian, Y., Supangat, S., & Agustina, D. (2020). Prevalensi Resistensi Escherichia coli Terhadap Tetrasiklin yang di isolasi dari hati Ayam Broiler. Jurnal Ilmu Dan Teknologi Peternakan, 8(3), 304–309. https://doi.org/DOI: 10.33772/jitro.v8i3.17584
Van Boeckel, T. P., Brower, C., Gilbert, M., Grenfell, B. T., Levin, S. A., Robinson, T. P., Teillant, A., & Laxminarayan, R. (2015). Global trends in antimicrobial use in food animals. Proceedings of the National Academy of Sciences of the United States of America, 112(18), 5649–5654. https://doi.org/10.1073/pnas.1503
